a549 hace2 flag nd human embryonic kidney Search Results


a549 hace2 flag nd human embryonic kidney  (ATCC)
99
ATCC a549 hace2 flag nd human embryonic kidney
A549 Hace2 Flag Nd Human Embryonic Kidney, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a549+hace2+flag+nd+human+embryonic+kidney/293T/10__1016_slash_j__fmre__2025__07__007-62-3-9
Average 99 stars, based on 1 article reviews
a549 hace2 flag nd human embryonic kidney - by Bioz Stars, 2026-09
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human lung carcinoma a549 hace2-a549 cell lines  (BEI Resources)
90
BEI Resources human lung carcinoma a549 hace2-a549 cell lines
In vitro characterization of the WT and ΔORF rSARS-CoV-2s. (A) Plaque phenotype. Vero E6 cells (6-well plate format, 10 6 cells/well) were infected with the WT, ORFΔ3a, ORFΔ6, ORFΔ7a, ORFΔ7b, or ORFΔ8 rSARS-CoV-2 and overlaid with medium containing agar. Plates were incubated at 37°C, and monolayers were immunostained with an anti-N protein SARS-CoV cross-reactive monoclonal antibody, 1C7C7, at the indicated hours p.i. (B) Viral plaque sizes. Diameters of viral plaques were measured with a ruler in centimeters. Plaques less than 0.1 cm are indicated as not detected (ND). (C to E) Multicycle growth kinetics. Vero E6 (C), <t>hACE2-HEK293T</t> (D), and <t>hACE2-A549</t> (E) cells (6-well plate format, 10 6 cells/well, triplicates) were infected (MOI 0.01) with the WT or a ΔORF rSARS-CoV-2 and incubated at 37°C. At the indicated hours p.i., tissue culture supernatants from infected cells were collected, viral titers were determined by plaque assay (PFU per milliliter), and cells were immunostained using the anti-N SARS-CoV cross-reactive monoclonal antibody 1C7C7. Data are the means ± standard deviations (SDs) of the results determined from triplicate wells. Dotted black lines indicate the limit of detection (LOD; 100 PFU/ml). * , P < 0.05, using the Student t test. ns, not significant.
Human Lung Carcinoma A549 Hace2 A549 Cell Lines, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a549+hace2+flag+nd+human+embryonic+kidney/a549+hace2/pmc08354228-127-16-24
Average 90 stars, based on 1 article reviews
human lung carcinoma a549 hace2-a549 cell lines - by Bioz Stars, 2026-09
90/100 stars
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a549/ace2 cells  (BEI Resources)
90
BEI Resources a549/ace2 cells
In vitro characterization of the WT and ΔORF rSARS-CoV-2s. (A) Plaque phenotype. Vero E6 cells (6-well plate format, 10 6 cells/well) were infected with the WT, ORFΔ3a, ORFΔ6, ORFΔ7a, ORFΔ7b, or ORFΔ8 rSARS-CoV-2 and overlaid with medium containing agar. Plates were incubated at 37°C, and monolayers were immunostained with an anti-N protein SARS-CoV cross-reactive monoclonal antibody, 1C7C7, at the indicated hours p.i. (B) Viral plaque sizes. Diameters of viral plaques were measured with a ruler in centimeters. Plaques less than 0.1 cm are indicated as not detected (ND). (C to E) Multicycle growth kinetics. Vero E6 (C), <t>hACE2-HEK293T</t> (D), and <t>hACE2-A549</t> (E) cells (6-well plate format, 10 6 cells/well, triplicates) were infected (MOI 0.01) with the WT or a ΔORF rSARS-CoV-2 and incubated at 37°C. At the indicated hours p.i., tissue culture supernatants from infected cells were collected, viral titers were determined by plaque assay (PFU per milliliter), and cells were immunostained using the anti-N SARS-CoV cross-reactive monoclonal antibody 1C7C7. Data are the means ± standard deviations (SDs) of the results determined from triplicate wells. Dotted black lines indicate the limit of detection (LOD; 100 PFU/ml). * , P < 0.05, using the Student t test. ns, not significant.
A549/Ace2 Cells, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a549+hace2+flag+nd+human+embryonic+kidney/vero+e6+tmprss2+t2a+ace2+cells/pmc09420051-51-2-3
Average 90 stars, based on 1 article reviews
a549/ace2 cells - by Bioz Stars, 2026-09
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vero c1008  (ATCC)
99
ATCC vero c1008
In vitro characterization of the WT and ΔORF rSARS-CoV-2s. (A) Plaque phenotype. Vero E6 cells (6-well plate format, 10 6 cells/well) were infected with the WT, ORFΔ3a, ORFΔ6, ORFΔ7a, ORFΔ7b, or ORFΔ8 rSARS-CoV-2 and overlaid with medium containing agar. Plates were incubated at 37°C, and monolayers were immunostained with an anti-N protein SARS-CoV cross-reactive monoclonal antibody, 1C7C7, at the indicated hours p.i. (B) Viral plaque sizes. Diameters of viral plaques were measured with a ruler in centimeters. Plaques less than 0.1 cm are indicated as not detected (ND). (C to E) Multicycle growth kinetics. Vero E6 (C), <t>hACE2-HEK293T</t> (D), and <t>hACE2-A549</t> (E) cells (6-well plate format, 10 6 cells/well, triplicates) were infected (MOI 0.01) with the WT or a ΔORF rSARS-CoV-2 and incubated at 37°C. At the indicated hours p.i., tissue culture supernatants from infected cells were collected, viral titers were determined by plaque assay (PFU per milliliter), and cells were immunostained using the anti-N SARS-CoV cross-reactive monoclonal antibody 1C7C7. Data are the means ± standard deviations (SDs) of the results determined from triplicate wells. Dotted black lines indicate the limit of detection (LOD; 100 PFU/ml). * , P < 0.05, using the Student t test. ns, not significant.
Vero C1008, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a549+hace2+flag+nd+human+embryonic+kidney/VERO+C1008/custom%40crl-1586%4034133899
Average 99 stars, based on 1 article reviews
vero c1008 - by Bioz Stars, 2026-09
99/100 stars
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Image Search Results


In vitro characterization of the WT and ΔORF rSARS-CoV-2s. (A) Plaque phenotype. Vero E6 cells (6-well plate format, 10 6 cells/well) were infected with the WT, ORFΔ3a, ORFΔ6, ORFΔ7a, ORFΔ7b, or ORFΔ8 rSARS-CoV-2 and overlaid with medium containing agar. Plates were incubated at 37°C, and monolayers were immunostained with an anti-N protein SARS-CoV cross-reactive monoclonal antibody, 1C7C7, at the indicated hours p.i. (B) Viral plaque sizes. Diameters of viral plaques were measured with a ruler in centimeters. Plaques less than 0.1 cm are indicated as not detected (ND). (C to E) Multicycle growth kinetics. Vero E6 (C), hACE2-HEK293T (D), and hACE2-A549 (E) cells (6-well plate format, 10 6 cells/well, triplicates) were infected (MOI 0.01) with the WT or a ΔORF rSARS-CoV-2 and incubated at 37°C. At the indicated hours p.i., tissue culture supernatants from infected cells were collected, viral titers were determined by plaque assay (PFU per milliliter), and cells were immunostained using the anti-N SARS-CoV cross-reactive monoclonal antibody 1C7C7. Data are the means ± standard deviations (SDs) of the results determined from triplicate wells. Dotted black lines indicate the limit of detection (LOD; 100 PFU/ml). * , P < 0.05, using the Student t test. ns, not significant.

Journal: Journal of Virology

Article Title: Contribution of SARS-CoV-2 Accessory Proteins to Viral Pathogenicity in K18 Human ACE2 Transgenic Mice

doi: 10.1128/JVI.00402-21

Figure Lengend Snippet: In vitro characterization of the WT and ΔORF rSARS-CoV-2s. (A) Plaque phenotype. Vero E6 cells (6-well plate format, 10 6 cells/well) were infected with the WT, ORFΔ3a, ORFΔ6, ORFΔ7a, ORFΔ7b, or ORFΔ8 rSARS-CoV-2 and overlaid with medium containing agar. Plates were incubated at 37°C, and monolayers were immunostained with an anti-N protein SARS-CoV cross-reactive monoclonal antibody, 1C7C7, at the indicated hours p.i. (B) Viral plaque sizes. Diameters of viral plaques were measured with a ruler in centimeters. Plaques less than 0.1 cm are indicated as not detected (ND). (C to E) Multicycle growth kinetics. Vero E6 (C), hACE2-HEK293T (D), and hACE2-A549 (E) cells (6-well plate format, 10 6 cells/well, triplicates) were infected (MOI 0.01) with the WT or a ΔORF rSARS-CoV-2 and incubated at 37°C. At the indicated hours p.i., tissue culture supernatants from infected cells were collected, viral titers were determined by plaque assay (PFU per milliliter), and cells were immunostained using the anti-N SARS-CoV cross-reactive monoclonal antibody 1C7C7. Data are the means ± standard deviations (SDs) of the results determined from triplicate wells. Dotted black lines indicate the limit of detection (LOD; 100 PFU/ml). * , P < 0.05, using the Student t test. ns, not significant.

Article Snippet: The human embryonic kidney 293T (HEK293T) and the human lung carcinoma (A549) cell lines stably expressing hACE2 (hACE2-HEK293T and hACE2-A549, respectively) were obtained from BEI Resources (NR-52511 and NR-53821, respectively).

Techniques: In Vitro, Infection, Incubation, Plaque Assay

Infection of K18 hACE2 transgenic mice with the WT or a ΔORF rSARS-CoV-2. Six- to 8-week-old K18 hACE2 transgenic female mice were mock (PBS) infected or infected (i.n.) with 10 5 PFU of the WT or a ΔORF rSARS-CoV-2 ( n = 4/group). Body weight (A) and survival (B) were evaluated at the indicated days p.i. Mice that lost >25% of their initial body weight were humanely euthanized. Error bars represent the SDs of the mean for each group.

Journal: Journal of Virology

Article Title: Contribution of SARS-CoV-2 Accessory Proteins to Viral Pathogenicity in K18 Human ACE2 Transgenic Mice

doi: 10.1128/JVI.00402-21

Figure Lengend Snippet: Infection of K18 hACE2 transgenic mice with the WT or a ΔORF rSARS-CoV-2. Six- to 8-week-old K18 hACE2 transgenic female mice were mock (PBS) infected or infected (i.n.) with 10 5 PFU of the WT or a ΔORF rSARS-CoV-2 ( n = 4/group). Body weight (A) and survival (B) were evaluated at the indicated days p.i. Mice that lost >25% of their initial body weight were humanely euthanized. Error bars represent the SDs of the mean for each group.

Article Snippet: The human embryonic kidney 293T (HEK293T) and the human lung carcinoma (A549) cell lines stably expressing hACE2 (hACE2-HEK293T and hACE2-A549, respectively) were obtained from BEI Resources (NR-52511 and NR-53821, respectively).

Techniques: Infection, Transgenic Assay

Replication of the WT and ΔORF rSARS-CoV-2s in nasal turbinate and lungs. Six- to 8-week-old K18 hACE2 transgenic female mice were mock (PBS) infected or infected (i.n.) with 10 5 PFU of the WT or a ΔORF rSARS-CoV-2 ( n = 8/group). Mice were sacrificed at 2 ( n = 4/group) and 4 ( n = 4/group) days p.i., and viral titers in nasal turbinate and lung were determined by plaque assay (PFU per milliliter) and immunostaining using the cross-reactive SARS-CoV 1C7C7 N protein monoclonal antibody. Viral titers in the nasal turbinate (A) and lungs (B) are shown. Symbols represent data from individual mice and bars the geometric means of viral titers. Dotted lines indicate the LOD (10 PFU/ml). ND, not detected; @, not detected in 1 mouse; #, not detected in 2 mice: &, not detected in 3 mice. Negative results of the PBS-infected mice are not plotted.

Journal: Journal of Virology

Article Title: Contribution of SARS-CoV-2 Accessory Proteins to Viral Pathogenicity in K18 Human ACE2 Transgenic Mice

doi: 10.1128/JVI.00402-21

Figure Lengend Snippet: Replication of the WT and ΔORF rSARS-CoV-2s in nasal turbinate and lungs. Six- to 8-week-old K18 hACE2 transgenic female mice were mock (PBS) infected or infected (i.n.) with 10 5 PFU of the WT or a ΔORF rSARS-CoV-2 ( n = 8/group). Mice were sacrificed at 2 ( n = 4/group) and 4 ( n = 4/group) days p.i., and viral titers in nasal turbinate and lung were determined by plaque assay (PFU per milliliter) and immunostaining using the cross-reactive SARS-CoV 1C7C7 N protein monoclonal antibody. Viral titers in the nasal turbinate (A) and lungs (B) are shown. Symbols represent data from individual mice and bars the geometric means of viral titers. Dotted lines indicate the LOD (10 PFU/ml). ND, not detected; @, not detected in 1 mouse; #, not detected in 2 mice: &, not detected in 3 mice. Negative results of the PBS-infected mice are not plotted.

Article Snippet: The human embryonic kidney 293T (HEK293T) and the human lung carcinoma (A549) cell lines stably expressing hACE2 (hACE2-HEK293T and hACE2-A549, respectively) were obtained from BEI Resources (NR-52511 and NR-53821, respectively).

Techniques: Transgenic Assay, Infection, Plaque Assay, Immunostaining

Gross pathology analysis of lungs from K18 hACE2 transgenic mice infected with the WT or a ΔORF rSARS-CoV-2. Lungs from 6-to-8-week-old female K18 hACE2 transgenic mice ( n = 8/group) mock (PBS) infected or infected (i.n.) with 10 5 PFU of the WT or a ΔORF rSARS-CoV-2 were harvested at 2 ( n = 4/group) or 4 ( n = 4/group) days p.i., imaged (A), and scored for virally induced lesions (B). The total lung surface area affected by virally induced lesions were determined. Scale bars, 3 cm.

Journal: Journal of Virology

Article Title: Contribution of SARS-CoV-2 Accessory Proteins to Viral Pathogenicity in K18 Human ACE2 Transgenic Mice

doi: 10.1128/JVI.00402-21

Figure Lengend Snippet: Gross pathology analysis of lungs from K18 hACE2 transgenic mice infected with the WT or a ΔORF rSARS-CoV-2. Lungs from 6-to-8-week-old female K18 hACE2 transgenic mice ( n = 8/group) mock (PBS) infected or infected (i.n.) with 10 5 PFU of the WT or a ΔORF rSARS-CoV-2 were harvested at 2 ( n = 4/group) or 4 ( n = 4/group) days p.i., imaged (A), and scored for virally induced lesions (B). The total lung surface area affected by virally induced lesions were determined. Scale bars, 3 cm.

Article Snippet: The human embryonic kidney 293T (HEK293T) and the human lung carcinoma (A549) cell lines stably expressing hACE2 (hACE2-HEK293T and hACE2-A549, respectively) were obtained from BEI Resources (NR-52511 and NR-53821, respectively).

Techniques: Transgenic Assay, Infection

(A) The cytokine and chemokine storms in the lungs of K18 hACE2 transgenic mice mock infected and infected with the WT or a ΔORF rSARS-CoV-2 were determined using an 8-plex panel mouse ProcartaPlex assay. (B) The IL-6/IL-10 ratio as a marker of the local cytokine storm induced by rSARS-CoV-2 was determined. A two-way ANOVA of K18 hACE2 transgenic mice mock infected or infected with the WT or an ORF-deficient rSARS-CoV-2 was carried out with 4 mice per time point, except for the mock-infected group ( n = 2).

Journal: Journal of Virology

Article Title: Contribution of SARS-CoV-2 Accessory Proteins to Viral Pathogenicity in K18 Human ACE2 Transgenic Mice

doi: 10.1128/JVI.00402-21

Figure Lengend Snippet: (A) The cytokine and chemokine storms in the lungs of K18 hACE2 transgenic mice mock infected and infected with the WT or a ΔORF rSARS-CoV-2 were determined using an 8-plex panel mouse ProcartaPlex assay. (B) The IL-6/IL-10 ratio as a marker of the local cytokine storm induced by rSARS-CoV-2 was determined. A two-way ANOVA of K18 hACE2 transgenic mice mock infected or infected with the WT or an ORF-deficient rSARS-CoV-2 was carried out with 4 mice per time point, except for the mock-infected group ( n = 2).

Article Snippet: The human embryonic kidney 293T (HEK293T) and the human lung carcinoma (A549) cell lines stably expressing hACE2 (hACE2-HEK293T and hACE2-A549, respectively) were obtained from BEI Resources (NR-52511 and NR-53821, respectively).

Techniques: Transgenic Assay, Infection, Marker